Dr inż. Katarzyna Wolny-Koładka

Dr inż. Mateusz Malinowski

Inż. Barbara Blok

Occurrence of selected soil microorganisms in points located by the main transport routes of Krakow

The research was conducted to determine the number and species composition of microorganisms isolated from the soils collected in the vicinity of seven largest transport nodes and roundabouts in Krakow. Moreover, the investigations aimed at verifying the differences in the occurrence and biodiversity of the researched microorganism population between four calendar seasons of the year. The soil samples were collected 4 times during the period from November 2013 to August 2014 and then analysed using serial dilutions method. A great microorganism biodiversity was found in the analysed samples. Microorganisms were the most numerous in the autumn-winter period. The most numerous isolated group were vegetative bacteria and ammonifiers. Filamentous fungi were less numerous, which may have been caused by the neutral or slightly alkaline soil pH. Presence of dormant bacteria forms may result from unfavourable environmental conditions caused by a toxic effect of the substances from road transport. Less numerously isolated were actinomycetes and Azotobacter bacteria, regarded as bioindicators of soil fertility. It was found, that the differences in the numbers of the analysed microorganisms over the year are statistically significant for the vegetative and ammonifying bacteria, phytopathogenic fungi and actinomycetes. No significant differences in the microorganism number were stated ...

Dr inż. Katarzyna Wolny-Koładka

Dr inż. Mateusz Malinowski

Agnieszka Sikora

Kamil Szymonik

Mgr Grzegorz Pelczar

Dr Katarzyna Wawrzyniak-Turek

THE EFFECT OF THE INTENSIVE AEROBIC BIOSTABILIZATION PHASE ON SELECTED MICROBIOLOGICAL AND PHYSICOCHEMICAL PARAMETERS OF WASTES

One of the most frequently applied methods of mixed municipal solid waste biological treatment in mechanical-biological treatment installations (MBT) is their disposal in aerobic biostabilization process. The process comprises an intensive phase and maturation phase. The intensive phase relies on waste heating in result of organic matter breakdown conducted by microorganisms settling the wastes. Microorganisms living in wastes have optimal conditions for development, i.e. a considerable space volume, optimal material fragmentation and organic matter availability. The aim of the aerobic biostabilization process is stabilizing and hygienization of wastes, so that they become a valuable raw material for other recovery processes (e.g. RDF production) or their disposal is safe for the environment and do not pose any epidemiological hazard for people employed in waste treatment plants.Analyses presented in the paper aimed to determine the number and species composition of vegetative and endospore bacteria, mold fungi, actinomycetes and pathogenic microorganisms, i.e. Staphylococcus spp., E. coli, Salmonella spp., Shigella spp., E. faecalis, C. perfringens, settling municipal wastes prior to and after aerobic stabilization process. The aerobic stabilization process (intensive phase) was conducted in a laboratory BKB 100 bioreactor using the wastes, characterized by low share of biodegradable wastes (<40 %), obtained from MBT ...

Dr inż. Katarzyna Wolny-Koładka

Inż. Agnieszka Sikora

Dr inż. Dagmara Malina

EVALUATION OF SILVER NANOPARTICLES TOXICITY TO DRUG-RESISTANT ESCHERICHIA COLI STRAINS ISOLATED FROM MUNICIPAL WASTE

The aim of the study was to evaluate the bactericidal properties of silver nanoparticles against bacteria Escherichia coli. In addition, a sensitivity analysis was made of E. coli to commonly used antibiotics. In the study were used 51 strains isolated from municipal waste. Applied growing concentrations of nanosilver were used: 5, 10, 15, 30, 60 and 125 ppm. The positive control was an antibiotic - ampicillin, negative control - a distilled water. Drug-resistance analysis was performed by disc-diffusion method. Along with the increase in the concentration of silver nanoparticles their antibacterial properties is also growing. The smallest concentration that inhibited growth of some isolates was 5 ppm, while the most effective concentration was 125 ppm. Analysis of drug resistance showed that the antibiotic inhibits growth of a large number of E. coli strains was amikacin. In addition, 22% of all isolates analyzed were drug-resistant. Nanosilver has a high bactericidal potential and may be helpful in solving the problem of drug resistance. However, it is reasonable to conduct further studies on the effects of toxicity of nanoparticles on the environment and higher organisms. ...

Dr inż. Mateusz Malinowski

Dr inż. Katarzyna Wolny-Koładka

Inż. Bartłomiej Jastrzębski

Characteristics of illegal dumping sites – case study: watercourses

Illegal waste deposition in places unadapted for this purpose poses a hazard to the natural environment. Each year over 10 000 illegal dumping sites are liquidated in Poland. The areas where illegal landfills occur are most frequently situated in the vicinity of waste management plants, wastelands, roadsides, byways or unfenced private plots (particularly on the city outskirts), forests and water courses. Despite of the changes introduced in recent years in the system of municipal solid waste management in Poland, which in the first place aimed to prevent formation of illegal dumps, such places still arise causing pollutant emission into the atmosphere. The aim of the paper was conducting an analysis of the existing illegal dumping sites in Krosno commune (Podkarpackie voivodship). The investigations were conducted along the selected stretches of the Wisłok and Lubatówka rivers. The analyses were carried out in three measurement series during the period from March to October 2014. As results from the analyses, new illegal dumping sites arise all the time in the water courses in Krosno city and a majority of them can be easily identified in this area. Glass and plastics have the biggest share in the morphological composition of the waste deposited on ...