The aim of the study was to evaluate the bactericidal properties of silver nanoparticles against bacteria Escherichia coli. In addition, a sensitivity analysis was made of E. coli to commonly used antibiotics. In the study were used 51 strains isolated from municipal waste. Applied growing concentrations of nanosilver were used: 5, 10, 15, 30, 60 and 125 ppm. The positive control was an antibiotic - ampicillin, negative control - a distilled water. Drug-resistance analysis was performed by disc-diffusion method. Along with the increase in the concentration of silver nanoparticles their antibacterial properties is also growing. The smallest concentration that inhibited growth of some isolates was 5 ppm, while the most effective concentration was 125 ppm. Analysis of drug resistance showed that the antibiotic inhibits growth of a large number of E. coli strains was amikacin. In addition, 22% of all isolates analyzed were drug-resistant. Nanosilver has a high bactericidal potential and may be helpful in solving the problem of drug resistance. However, it is reasonable to conduct further studies on the effects of toxicity of nanoparticles on the environment and higher organisms. ...
The aim of the study was to assess the drug resistance profile of E. coli bacteria isolated from municipal waste. It was visible whether among the collected isolates there are strains resistant to ESBL mechanisms (extended spectrum β-lactamase) and whether they are resistant to β-lactam antibiotics. Analysis of the use of disk diffusion methods, using 7 final results for β-lactams (including 2 penicillins, 5 cephalosporins). The obtained comparative results with the KORLD (National Reference Center for Antimicrobial Susceptibility) and EUCAST (European Committee for Antimicrobial Susceptibility Testing) standards. The analysis distinguished that among 70% of isolates there is no resistance marking to unconfirmed from the tested antibiotics. On the contrary, 30% of strains were resistant to more than 1 antibiotic. Intermediate susceptibility to cefazolin is characteristic of 80% of E. coli strains. The largest number, as many as 20% of strains, were resistant to this antibiotic. In contrast to 100% susceptibility compared to amoxicillin with clavulanic acid, which should be used for penicillin. The ESBL mechanism is not a habitat. It has been found that municipal waste is a place of residence of E. coli bacteria resistant to antibiotics. It is very important to continue research on this source of drug ...
